DyLight peptides

DyLight™ Dyes - high performance dyes for the most demanding applications.

Alta Bioscience is pleased to be able to offer peptides labelled with the DyLight™ range of fluorophores. Pierce Technologies have developed the nine DyLight™ compounds, which are a family of fluorescent molecules showing very high photostability and fluorescence intensity. These dyes are ideally suited for the most demanding experiments where the highest performing fluorophores are essential.

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Properties of the DyLight™ dyes

Dye	Emission colour	Abs	Em	Extinction coefficient*	Similar dyes
DyLight 405	Blue	400nm	420nm	30x10³	Alexa 405, Cascade blue
DyLight 488	Green	493nm	518nm	70x10³	Alexa 488, FAM
DyLight 549	Yellow	550nm	568nm	150x10³	Alexa 546, Alexa 555, Cy3
DyLight 594	Red	593nm	618nm	80x10³	Alexa 594, Texas red
DyLight 633	Red	638nm	658nm	170x10³	Alexa 633
DyLight 649	Red	646nm	674nm	250x10³	Alexa 647, Cy5
DyLight 680	Near IR	682nm	715nm	140x10³	Alexa 680, Cy5.5
DyLight 750	Near IR	752nm	778nm	210x10³	Alexa 750
DyLight 800	Infrared	770nm	794nm	270x10³	IRDye 800

* units of M^-1 cm^-1 at the λ max

The primary advantages of these dyes are:-

A wide spectral range.
The dyes cover the excitation range of 400nm to 770nm, spanning the principle wavelengths used by most common instrumentation.
Photo stability and intensity.
In many applications, the DyLight™ dyes show higher photo stability and fluorescence intensity than either the Alexa Fluor™ or the CyDyes™ .
Wide usable pH range.
They remain highly fluorescent over a wide pH range, (pH4–pH9).
Good water solubility
Peptides synthesised with DyLight™ dyes can be readily solubilised.
Narrow emission spectra
This enables the use of multiple dyes with minimum signal crossover.

The dyes can be incorporated at any position in a peptide, although the N-terminus is the most cost effective site.

The DyLight-peptides are supplied lyophilised in amber glass vials.
HPLC and MALDI traces are supplied with each product.

Protein labelling service
In addition to the dye-peptide products, Alta Bioscience is offering a protein labelling service, tagging your protein with the DyLight™ Dye of your choice.

Please contact us for more information on this operation.

Trade marks
DyLight™ is a trade mark of Pierce Biotechnology. Alexa Fluor™ is a trade mark of Molecular Probes. CyDye™ is a trade mark of GE Healthcare. IR Dye™ is a trade mark of LI-COR Biosciences.

Please note, that our DyLight™ labelled peptides and proteins are supplied for research use only and cannot be used for diagnosis, medical or veterinary applications.

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